All rights reserved. Calm demeanor Easy to handle in the lab However, a direct comparison between Wistar and Sprague-Dawley (SD) Rat as models of high-fat (HF) diet-induced obesity has not been adequately evaluated so far. 360 Sprague-Dawley rats (179 males and 181 females) and a 26% incidence was seen in 254 Swiss mice (101 males and 153 females) used as untreated control animals in an 18-month series of carcinogenesis experiments. A total of 30 male Sprague‑Dawley rats were randomly divided into three groups (n=10), including the control, OA and OA with treadmill exercise (OAE) groups. ASC transplantation significantly restored the mechanical threshold reduced by burn injury. It has not been formally disseminated by the NTP. 0000001646 00000 n In order to carry out comparative studies between animal models and humans, a precise phenotypic characterization should be performed, accounting for both muscle development and metabolism (Capitanio et al., 2005(Capitanio et al., , 2009, ... Ectopic secretion of lysosomal proteases can, in fact, degradate extracellular matrix, contributing to atherosclerosis, arthritis, aneurysms formation, and other diseases [63]. Three of 105 untreated control animals developed carcinomas of the sebaceous gland (Zymbal) of the external auditory canal at an average age of 115 weeks. MKK7 mRNA and p-MKK7 levels were decreased in the 3 months group after Sunitinib treatment. Read Online PDF (2 MB) ... For the first time, this study treated Sprague Dawley (SD) rats with 3-MCPD 1-monooleate at 10 and 100 mg/kg BW/day and 1-monostearate at 15 and 150 mg/kg BW/day for 90 days and examined for their potential semi-long-term nephrotoxicity and the associated molecular mechanisms. The Sprague Dawley rat is an outbred multipurpose breed of albino rat used extensively in medical research. 0000010274 00000 n Treatment was done by oral administration of extract at doses of 1 mL, 2.5 mL and 5 mL. Alterations in articular cartilage and synovium were also evaluated by histology, immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction. The percentage of female rats with tumors was almost injection of pentylenetetrazol (PTZ, 70–80 mg kg −1); some pups of each litter were used as controls. These patients (52 men and 11 women, with age range 49-72 years) were randomized into two groups: untreated group (n = 30) and α-LA group (n = 33). Interleukin (IL)‑1β and IL‑4 levels in the serum and intra‑articular lavage fluid (IALF) were measured by ELISA. In rat hearts, 6 stress response and 4 other proteins were identified: Gstp1, glutathione S-transferase P; Hspa8, heat shock cognate 71kDa protein; Mpst, 3-mercaptopyruvate sulfurtransferase; Pdia3, protein disulfide-isomerase A3; Prdx2, peroxiredoxin-2; Rnh1, ribonuclease inhibitor; Anxa5, annexin A5; Serpina1, alpha-1-antiproteinase (2 proteoforms); Serpina3k, serine protease inhibitor A3K; Serpina3l, serine protease inhibitor A3L. Sprague Dawley rats; Pharmacological interventions; Toxicological effects. Reduction in function of extra-cardiac organs is likely to be another important mechanism by which heart failure increases with aging, since heart failure is a multiple organ system disease. Conversely, the effects of aging in Col6a1−/− gastrocnemius were similar but delayed becoming apparent at 12 months of age. A designed behavior test was applied for pain evaluation. Cancer Chemotherapy and Pharmacology 2017, 80 (2) , 317-332. The rats were refreshed with NIH Genetic Resource stock in 1998. Serpins in the mammalian body are highly potent serine protease inhibitors which modulate both thrombotic and thrombolytic pathway activation, with direct and indirect crosstalk with immune and inflammatory pathways. Literature data show beneficial effects of exercise on the impairment of mitochondrial biogenesis and dynamics, and on the decrease in the mitophagic capacity associated to aging. Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard (St) and high-fat (HF) diet groups. Table 1. In the skeletal muscle, the ageing process is characterized by a loss of muscle mass and strength, coupled with a decline of mitochondrial function and a decrease of satellite cells. Protein kinase A treatment abolished basal differences in rates of cross-bridge recruitment between MyBP-C(+/-) and wild-type myocardium. Recent advances in basic science clarify several important mechanisms of aging. To elucidate the effects of decreased MyBP-C expression on cardiac function, we employed MyBP-C heterozygous null (MyBP-C(+/-)) mice presenting similar decreases in MyBP-C expression (32%) as FHC patients carrying MyBP-C mutations. 2-AG signaling depends on the type of neurons involved in the process (4, 13–16). An isoprostane, 8-iso-prostaglandin F2α (8-iso-PGF2α), one product of arachidonic acid metabolism, was measured as a marker for oxidative stress. In this study, we have investigated the effects of ROS on actin in an established rat model of acute oxidative stress using short-term X-ray irradiation. The studies discussed range from thrombosis and hemorrhage to vascular disease and transplant rejection and finally to sepsis and clinical studies in humans. I am a model organism used by Duke University to research the impacts of chemical exposure on behavior, memory, and cognitive ability. Upper panel: Histograms of protein expression normalized to the total protein stain (mean±SD). However, most studies have been focused on skeletal muscle features. In diaphragm, the differences displayed by 6-month-old Col6a1−/− mice were more pronounced compared to wild-type mice and persisted at 12 months of age. Access scientific knowledge from anywhere. At 24 hours and 1 week after treatment, ALDH2 activity was significantly higher in the α-LA group than in the untreated group (P < 0.05), whereas the levels of 8-iso-PGF2α were significantly lower in the α-LA group than in the untreated group (all P < 0.05). Reproductive Toxicology Impairment in Male Sprague-Dawley Rats BinSong, 1,2,3 FengjuanWang, 4 andWeiWang 1,2,3 Department of Human Anatomy and Histoembryology, School of Basic Medical Sciences, Fujian Medical University, However, the mechanism by which heart function declines and heart failure increases in association with age is not clear. Among the four tryptophan residues present in actin, only Trp(79) and Trp(86) appeared to undergo oxidation. Statistical analysis was performed by Student’s T-Test (n=3; * p<0.05). The rats are maintained as an outbred closed colony. Anti-cancer drug Sunitinib is linked to adverse cardiovascular events, which have shown to involve mitogen activated kinase kinase 7 (MKK7) pathway. All rights reserved. To Charles River in 1996. Frequently Asked Questions 1. The majority of MyBP-C mutations are expected to reduce MyBP-C expression, however, the consequences of MyBP-C deficiency on the regulation of myofilament function, Ca(2+) homeostasis, and in vivo cardiac function are unknown. Albino rats (Rattusnorvegicus; Sprague-Dawley The experiment animals were marked carefully on 147 Rakib Hasan, et al. High levels of dihydrolipoyllysine-residue acetyltransferase (Dlat) and ATP synthase subunit alpha (Atp5a1) are detected in triceps and gastrocnemius, respectively. B) Validation of Hspa8 protein by immunoblotting in young, old adult and senescent rat cardiac muscles. These results reinforce the possibility that OPA1 might act as an inhibitor of mitochondrial fission [64]. To address reports of TBP accumulation in human placenta and breast milk, studies were conducted to characterize TBP disposition and toxicokinetics in timed-pregnant or nursing Sprague Dawley (SD) rats following a single oral dose to the dam. The percentage of female rats with tumors was almost double that of males, which difference was accounted for chiefly by the high incidence of mammary tumors in the females. November 2018 . 0000003517 00000 n The wild-type Sprague Dawley rat thymus was comprised mostly of CD4/CD8 double positive cells whereas in the SRG rat, the viable cells recovered were CD4-/CD8- (Fig 1A–1C). Statistical analysis was performed by Student’s T-Test (n=3; * p<0.05). Furthermore, two relevant genes of the model have been also validated by Western blot experiments on heart samples from young and old mice, confirming in vitro their ectopic expression in aged hearts. Incidence of neoplastic lesions in untreated Sprague-Dawley rats (1340 males and 1329 females) used as controls in 17 carcinogenicity studies are tabulated and evaluated. 0000015139 00000 n The percentage of female rats with tumors was almost double that of males, which difference was accounted for chiefly … Sprague Dawley rat Hello! Laboratory Animals, 24, 253-264 (1990). 0000001221 00000 n We analyzed the therapeutic efficacy of α-LA in 63 patients with confirmed acute coronary syndrome (ACS). The results revealed that IL‑1β in the serum and IALF decreased in the OAE group, whereas IL‑4 increased, and histological evaluation indicated that the OAE group had a clear treatment response. The Wistar rat is currently one of the most popular rats used for laboratory research. Sprague–Dawley rats, born in the laboratory.were maintained under conditions of controlled temperature and lighting (12 h-12 h) with free access to food and water. This paper. The Hsd:Sprague Dawley ® SD rat has a wide array of historical research use including in the field of neuroscience. 0000021021 00000 n Biochemical analysis of mitochondrial plasticity. Frequently Asked Questions 1. However, a direct comparison between Wistar and Sprague-Dawley (SD) Rat as models of high-fat (HF) diet-induced obesity has not been adequately evaluated so far. Blood, 56 (5) (1980). It is the most widely used rat model for biomedical research. 0000000896 00000 n The Sprague Dawley® outbred model was developed by Sprague Dawley, Inc. NIH received stock from Sprague Dawley, Inc in 1945.

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